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ah136b tumour (rat hepatoma) cells  (SLC Inc)

 
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    SLC Inc ah136b tumour (rat hepatoma) cells
    Apoptosis induction and change in HO activity after treatment with ZnPP IX of <t>AH136B</t> solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.
    Ah136b Tumour (Rat Hepatoma) Cells, supplied by SLC Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ah136b+tumour+%28rat+hepatoma%29+cells/pmc02377071-8-0-23?v=SLC+Inc
    Average 90 stars, based on 1 article reviews
    ah136b tumour (rat hepatoma) cells - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour"

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6600830

    Apoptosis induction and change in HO activity after treatment with ZnPP IX of AH136B solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.
    Figure Legend Snippet: Apoptosis induction and change in HO activity after treatment with ZnPP IX of AH136B solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Techniques Used: Activity Assay, TUNEL Assay, Staining, Control

    Zinc protoporphyrin IX-induced apoptosis of AH136B cells in vitro . AH136B cells were incubated for 24 h with indicated concentrations of ZnPP IX, with or without caspase-3 inhibitor ( A ) or SnPP IX ( B ), or with 100 μ M CuPP IX. ( A, B ) TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs control ( n =3 for each group). Cell viability after the same treatment is shown in the inset. ** P < 0.05, ** P <0.01 vs control ( n =4 for each group). Data are means±s.e. ( C ) Representative TUNEL staining of control and ZnPP IX-treated tumour cells. See text for details.
    Figure Legend Snippet: Zinc protoporphyrin IX-induced apoptosis of AH136B cells in vitro . AH136B cells were incubated for 24 h with indicated concentrations of ZnPP IX, with or without caspase-3 inhibitor ( A ) or SnPP IX ( B ), or with 100 μ M CuPP IX. ( A, B ) TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs control ( n =3 for each group). Cell viability after the same treatment is shown in the inset. ** P < 0.05, ** P <0.01 vs control ( n =4 for each group). Data are means±s.e. ( C ) Representative TUNEL staining of control and ZnPP IX-treated tumour cells. See text for details.

    Techniques Used: In Vitro, Incubation, TUNEL Assay, Control, Staining

    Effect of ZnPP IX on HO activity and caspase-3 activity of AH136B cells cultured in vitro . Haem oxygenase activity was measured 24 h after incubation with vehicle (control), ZnPP IX, or CuPP IX ( A ). Similarly, cells were treated with 100 μ M ZnPP with or without caspase-3 inhibitor (1 or 10 μ M ) for 24 h ( B ). Caspase-3 activity was measured fluorome-trically in cell extracts by using a fluorescent substrate. ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.
    Figure Legend Snippet: Effect of ZnPP IX on HO activity and caspase-3 activity of AH136B cells cultured in vitro . Haem oxygenase activity was measured 24 h after incubation with vehicle (control), ZnPP IX, or CuPP IX ( A ). Similarly, cells were treated with 100 μ M ZnPP with or without caspase-3 inhibitor (1 or 10 μ M ) for 24 h ( B ). Caspase-3 activity was measured fluorome-trically in cell extracts by using a fluorescent substrate. ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Techniques Used: Activity Assay, Cell Culture, In Vitro, Incubation, Control

    Protective effect of bilirubin against ZnPP IX-induced apoptosis of AH136B cells. AH136B cells were incubated for 24 h with 100 μ M ZnPP IX in the presence or absence of indicated concentrations of bilirubin. TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs ZnPP IX alone ( n =3 for each group). Data are means±s.e. See text for details.
    Figure Legend Snippet: Protective effect of bilirubin against ZnPP IX-induced apoptosis of AH136B cells. AH136B cells were incubated for 24 h with 100 μ M ZnPP IX in the presence or absence of indicated concentrations of bilirubin. TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs ZnPP IX alone ( n =3 for each group). Data are means±s.e. See text for details.

    Techniques Used: Incubation, TUNEL Assay

    Tumour blood flow after ZnPP IX or L -NAME treatment. Tumour blood flow was measured in AH136B solid tumour on day 14 after tumour implantation. At the indicated times after injection of 500 μ g kg −1 ZnPP IX (i.a.) or 6 mg kg −1 L -NAME (i.p.), a laser Doppler flowmeter and a probe needle were used to measure blood flow. Relative changes in tumour blood flow after injection of ZnPP IX or L -NAME are shown. Inset, change in tumour blood flow during early period after treatment (within 25 min). ** P <0.01 by ANOVA between the groups ( n =3 for each time point). Data are means±s.e. See text for details.
    Figure Legend Snippet: Tumour blood flow after ZnPP IX or L -NAME treatment. Tumour blood flow was measured in AH136B solid tumour on day 14 after tumour implantation. At the indicated times after injection of 500 μ g kg −1 ZnPP IX (i.a.) or 6 mg kg −1 L -NAME (i.p.), a laser Doppler flowmeter and a probe needle were used to measure blood flow. Relative changes in tumour blood flow after injection of ZnPP IX or L -NAME are shown. Inset, change in tumour blood flow during early period after treatment (within 25 min). ** P <0.01 by ANOVA between the groups ( n =3 for each time point). Data are means±s.e. See text for details.

    Techniques Used: Injection

    Western blot analysis of HSP70 and HO-1 proteins in AH136B cells and solid tumours. ( A ) Cells were incubated with SNAP (10 or 100 μ M ), P-NONOate (10 or 100 μ M ), or ZnPP IX (100 μ M ) for 6 h or, as a positive control, were treated by heat at 42°C for 30 min, followed by incubation at 37°C for 6 h. ( B ) AH136B solid tumours were treated with 0.9% NaCl solution (control), ZnPP IX (500 μ g kg −1 i.a.), L -NAME (6 mg kg −1 day −1 for 5 days i.p.), or SMT (6 mg kg −1 day −1 for 5 days i.p.). ( C ) Solid tumours were resected at 24 or 48 h after initiation of surgical occlusion of the common iliac artery, which serves the tumour-feeding artery of the tumour-implanted side. Control rats were treated by sham operation. Then, expression of HSP70 and HO-1 proteins was detected by Western blotting by using a monoclonal antibody to mammalian inducible HSP70 or a polyclonal antibody to rat HO-1. Each data point shown represents three independent experiments. See text for details.
    Figure Legend Snippet: Western blot analysis of HSP70 and HO-1 proteins in AH136B cells and solid tumours. ( A ) Cells were incubated with SNAP (10 or 100 μ M ), P-NONOate (10 or 100 μ M ), or ZnPP IX (100 μ M ) for 6 h or, as a positive control, were treated by heat at 42°C for 30 min, followed by incubation at 37°C for 6 h. ( B ) AH136B solid tumours were treated with 0.9% NaCl solution (control), ZnPP IX (500 μ g kg −1 i.a.), L -NAME (6 mg kg −1 day −1 for 5 days i.p.), or SMT (6 mg kg −1 day −1 for 5 days i.p.). ( C ) Solid tumours were resected at 24 or 48 h after initiation of surgical occlusion of the common iliac artery, which serves the tumour-feeding artery of the tumour-implanted side. Control rats were treated by sham operation. Then, expression of HSP70 and HO-1 proteins was detected by Western blotting by using a monoclonal antibody to mammalian inducible HSP70 or a polyclonal antibody to rat HO-1. Each data point shown represents three independent experiments. See text for details.

    Techniques Used: Western Blot, Incubation, Positive Control, Control, Expressing



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    ah136b tumour (rat hepatoma) cells  (SLC Inc)
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    SLC Inc ah136b tumour (rat hepatoma) cells
    Apoptosis induction and change in HO activity after treatment with ZnPP IX of <t>AH136B</t> solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.
    Ah136b Tumour (Rat Hepatoma) Cells, supplied by SLC Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ah136b+tumour+%28rat+hepatoma%29+cells/pmc02377071-8-0-23?v=SLC+Inc
    Average 90 stars, based on 1 article reviews
    ah136b tumour (rat hepatoma) cells - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Apoptosis induction and change in HO activity after treatment with ZnPP IX of AH136B solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Apoptosis induction and change in HO activity after treatment with ZnPP IX of AH136B solid. Apoptosis ( A, B ) and HO activity ( C ) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) ( A, a ), with ZnPP IX (500 μ g kg −1 i.a.) ( A, b ), or with CuPP IX (500 μ g kg −1 i.a.) ( A, c ). Quantitative analysis of TUNEL-positive cells in each specimen is shown ( B ). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm 2 was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis ( C ). ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: Activity Assay, TUNEL Assay, Staining, Control

    Zinc protoporphyrin IX-induced apoptosis of AH136B cells in vitro . AH136B cells were incubated for 24 h with indicated concentrations of ZnPP IX, with or without caspase-3 inhibitor ( A ) or SnPP IX ( B ), or with 100 μ M CuPP IX. ( A, B ) TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs control ( n =3 for each group). Cell viability after the same treatment is shown in the inset. ** P < 0.05, ** P <0.01 vs control ( n =4 for each group). Data are means±s.e. ( C ) Representative TUNEL staining of control and ZnPP IX-treated tumour cells. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Zinc protoporphyrin IX-induced apoptosis of AH136B cells in vitro . AH136B cells were incubated for 24 h with indicated concentrations of ZnPP IX, with or without caspase-3 inhibitor ( A ) or SnPP IX ( B ), or with 100 μ M CuPP IX. ( A, B ) TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs control ( n =3 for each group). Cell viability after the same treatment is shown in the inset. ** P < 0.05, ** P <0.01 vs control ( n =4 for each group). Data are means±s.e. ( C ) Representative TUNEL staining of control and ZnPP IX-treated tumour cells. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: In Vitro, Incubation, TUNEL Assay, Control, Staining

    Effect of ZnPP IX on HO activity and caspase-3 activity of AH136B cells cultured in vitro . Haem oxygenase activity was measured 24 h after incubation with vehicle (control), ZnPP IX, or CuPP IX ( A ). Similarly, cells were treated with 100 μ M ZnPP with or without caspase-3 inhibitor (1 or 10 μ M ) for 24 h ( B ). Caspase-3 activity was measured fluorome-trically in cell extracts by using a fluorescent substrate. ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Effect of ZnPP IX on HO activity and caspase-3 activity of AH136B cells cultured in vitro . Haem oxygenase activity was measured 24 h after incubation with vehicle (control), ZnPP IX, or CuPP IX ( A ). Similarly, cells were treated with 100 μ M ZnPP with or without caspase-3 inhibitor (1 or 10 μ M ) for 24 h ( B ). Caspase-3 activity was measured fluorome-trically in cell extracts by using a fluorescent substrate. ** P <0.05, ** P <0.01 vs control ( n =3 for each group). Data are means±s.e. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: Activity Assay, Cell Culture, In Vitro, Incubation, Control

    Protective effect of bilirubin against ZnPP IX-induced apoptosis of AH136B cells. AH136B cells were incubated for 24 h with 100 μ M ZnPP IX in the presence or absence of indicated concentrations of bilirubin. TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs ZnPP IX alone ( n =3 for each group). Data are means±s.e. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Protective effect of bilirubin against ZnPP IX-induced apoptosis of AH136B cells. AH136B cells were incubated for 24 h with 100 μ M ZnPP IX in the presence or absence of indicated concentrations of bilirubin. TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the percentage of positive cells was calculated. ** P <0.05 vs ZnPP IX alone ( n =3 for each group). Data are means±s.e. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: Incubation, TUNEL Assay

    Tumour blood flow after ZnPP IX or L -NAME treatment. Tumour blood flow was measured in AH136B solid tumour on day 14 after tumour implantation. At the indicated times after injection of 500 μ g kg −1 ZnPP IX (i.a.) or 6 mg kg −1 L -NAME (i.p.), a laser Doppler flowmeter and a probe needle were used to measure blood flow. Relative changes in tumour blood flow after injection of ZnPP IX or L -NAME are shown. Inset, change in tumour blood flow during early period after treatment (within 25 min). ** P <0.01 by ANOVA between the groups ( n =3 for each time point). Data are means±s.e. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Tumour blood flow after ZnPP IX or L -NAME treatment. Tumour blood flow was measured in AH136B solid tumour on day 14 after tumour implantation. At the indicated times after injection of 500 μ g kg −1 ZnPP IX (i.a.) or 6 mg kg −1 L -NAME (i.p.), a laser Doppler flowmeter and a probe needle were used to measure blood flow. Relative changes in tumour blood flow after injection of ZnPP IX or L -NAME are shown. Inset, change in tumour blood flow during early period after treatment (within 25 min). ** P <0.01 by ANOVA between the groups ( n =3 for each time point). Data are means±s.e. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: Injection

    Western blot analysis of HSP70 and HO-1 proteins in AH136B cells and solid tumours. ( A ) Cells were incubated with SNAP (10 or 100 μ M ), P-NONOate (10 or 100 μ M ), or ZnPP IX (100 μ M ) for 6 h or, as a positive control, were treated by heat at 42°C for 30 min, followed by incubation at 37°C for 6 h. ( B ) AH136B solid tumours were treated with 0.9% NaCl solution (control), ZnPP IX (500 μ g kg −1 i.a.), L -NAME (6 mg kg −1 day −1 for 5 days i.p.), or SMT (6 mg kg −1 day −1 for 5 days i.p.). ( C ) Solid tumours were resected at 24 or 48 h after initiation of surgical occlusion of the common iliac artery, which serves the tumour-feeding artery of the tumour-implanted side. Control rats were treated by sham operation. Then, expression of HSP70 and HO-1 proteins was detected by Western blotting by using a monoclonal antibody to mammalian inducible HSP70 or a polyclonal antibody to rat HO-1. Each data point shown represents three independent experiments. See text for details.

    Journal: British Journal of Cancer

    Article Title: Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid tumour

    doi: 10.1038/sj.bjc.6600830

    Figure Lengend Snippet: Western blot analysis of HSP70 and HO-1 proteins in AH136B cells and solid tumours. ( A ) Cells were incubated with SNAP (10 or 100 μ M ), P-NONOate (10 or 100 μ M ), or ZnPP IX (100 μ M ) for 6 h or, as a positive control, were treated by heat at 42°C for 30 min, followed by incubation at 37°C for 6 h. ( B ) AH136B solid tumours were treated with 0.9% NaCl solution (control), ZnPP IX (500 μ g kg −1 i.a.), L -NAME (6 mg kg −1 day −1 for 5 days i.p.), or SMT (6 mg kg −1 day −1 for 5 days i.p.). ( C ) Solid tumours were resected at 24 or 48 h after initiation of surgical occlusion of the common iliac artery, which serves the tumour-feeding artery of the tumour-implanted side. Control rats were treated by sham operation. Then, expression of HSP70 and HO-1 proteins was detected by Western blotting by using a monoclonal antibody to mammalian inducible HSP70 or a polyclonal antibody to rat HO-1. Each data point shown represents three independent experiments. See text for details.

    Article Snippet: AH136B tumour (rat hepatoma) cells were implanted subcutaneously (s.c.) in a dorsal site on the foot of male Donryu rats weighing 160–180 g (SLC, Inc., Shizuoka, Japan) with an inoculum size of 1 × 10 7 cells per injection site as described previously ( Doi et al , 1996 ).

    Techniques: Western Blot, Incubation, Positive Control, Control, Expressing